商品介绍
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against partial recombinant human AFP.
- Immunogen:
- Recombinant protein corresponding to amino acids 51-184 of human AFP.
- Sequence:
- FAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAAR
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Antigen affinity purification
- Isotype:
- IgG
- Recommend Usage:
- Immunofluorescence (1 - 4 ug/mL)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200 - 1:500)Western Blot (1:100 - 1:250)The optimal working dilution should be determined by the end user.
- Storage Buffer:
- In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
- Storage Instruction:
- Store at 4°C for short term storage. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Datasheet:
Download
- Applications
- Western Blot (Transfected lysate)
- Western Blot analysis with AFP polyclonal antibody (Cat # PAB30577).Lane 1: Negative control (vector only transfected HEK293T lysate)Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human placenta with AFP polyclonal antibody (Cat # PAB30577) shows extra cellular positivity.
- Immunofluorescence
- Immunofluorescent staining of human cell line U-251 MG with AFP polyclonal antibody (Cat # PAB30577) shows positivity in cytoplasm. Antibody staining is shown in green.
- Application Image
- Western Blot (Transfected lysate)
- enlarge
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- enlarge
- Immunofluorescence
- enlarge
- Gene Information
- Entrez GeneID:
- 174
- Protein Accession#:
- P02771
- Gene Name:
- AFP
- Gene Alias:
- FETA,HPAFP
- Gene Description:
- alpha-fetoprotein
- Omim ID:
- 104150
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq
- Other Designations:
- OTTHUMP00000160480,alpha-1-fetoprotein,alpha-fetoglobulin
- Related Disease
- Carcinoma, Hepatocellular
- Liver Cirrhosis
- Liver Neoplasms
品牌介绍
Abnova公司是专注于高通量蛋白表达和抗体制作，并将世界级的生物技术与台湾IT工业管理经验独特融于一体的全球性生物公司。Abnova通过基因组 学手段生产出的几万种重组蛋白和抗体，极大的扩展了生物试剂应用的视野和多样性。同时，Abnova还在不断的扩展其在系统应用中的产品线，包括成对抗 体、ELISA试剂盒、原位杂交荧光探针、无表达蛋白定量以及可用于蛋白翻译研究、诊断和制药公司应用的基础型体外诊断设备等。作为Abnova的授权代理商，艾美捷科技有限公司凭借丰富的专业经验，将一如既往的为中国区域客户提供Abnova优质的产品和技术服务。
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