商品介绍
- Specification
- Product Description:
- This protein phosphorylation antibody pair set comes with two antibodies, one against the BRAF protein, and the other against the specific S601 phosphorylated site of BRAF for use in in situ Proximity Ligation Assay. See Publication Reference below.
- Reactivity:
- Human
- Quality Control Testing:
- Dual recognition immunofluorescence result.
Representative image of Proximity Ligation Assay of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit polyclonal antibody 1:1200 and mouse monoclonal antibody 1:50. Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
- Supplied Product:
- Antibody pair set content: 1. Phospho-BRAF S601 rabbit polyclonal antibody (20 ul) With 0.09% sodium azide. 2. BRAF mouse monoclonal antibody (40 ug) In 1x PBS, pH 7.2 *Reagents are sufficient for at least 30-50 assays using recommended protocols.
- Storage Instruction:
- Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
- Datasheet:
Download
- Publication Reference
- 1.
- In situ detection of phosphorylated platelet-derived growth factor receptor beta using a generalized proximity ligation method.Jarvius M, Paulsson J, Weibrecht I, Leuchowius KJ, Andersson AC, Wahlby C, Gullberg M,Botling J,Sjoblom T, Markova B, Ostman A, Landegren U, Soderberg O.Mol Cell Proteomics. 2007 Sep;6(9):1500-9. Epub 2007 Jun 12.
- 2.
- Direct observation of individual endogenous protein complexes in situ by proximity ligation.Soderberg O, Gullberg M, Jarvius M , Ridderstrale K, Leuchowius KJ, Jarvius J, Wester K, Hydbring P, Bahram F, Larsson LG, and Landegren U. Nat Methods. 2006 Dec;3(12):995-1000. Epub 2006 Oct 29.
- 3.
- Cytokine detection by antibody-based proximity ligation.Gullberg M, Gustafsdottir SM, Schallmeiner E, Jarvius J, Bjarnegard M, Betsholtz C, Landegren U, andFredriksson S. Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8420-4. Epub 2004 May 21.
- 4.
- Protein detection using proximity-dependent DNA ligation assays.Fredriksson S, GullbergM, Jarvius J, Olsson C, Pietras K, Gustafsdottir SM, Ostman A, and Landegren U. Nat Biotechnol. 2002 May;20(5):473-7.
- 5.
- Highly specific detection of phosphorylated proteins by DuolinkMats Gullberg and Ann-Catrin Andersson Nature Methods 6. 2009
- Applications
- In situ Proximity Ligation Assay (Cell)
- Application Image
- In situ Proximity Ligation Assay (Cell)
- Gene Information
- Entrez GeneID:
- 673
- Gene Name:
- BRAF
- Gene Alias:
- B-RAF1,BRAF1,FLJ95109,MGC126806,MGC138284,RAFB1
- Gene Description:
- v-raf murine sarcoma viral oncogene homolog B1
- Omim ID:
- 115150, 164757, 211980
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq
- Other Designations:
- 94 kDa B-raf protein,B-Raf proto-oncogene serine/threonine-protein kinase (p94),Murine sarcoma viral (v-raf) oncogene homolog B1
- Gene Pathway
- Acute myeloid leukemia
- Bladder cancer
- Chemokine signaling pathway
- Chronic myeloid leukemia
- Colorectal cancer
- Endometrial cancer
- ErbB signaling pathway
- Focal adhesion
- Glioma
- Insulin signaling pathway
- Long-term depression
- Long-term potentiation
- MAPK signaling pathway
- Melanoma
- mTOR signaling pathway
- Natural killer cell mediated cytotoxicity
- Neurotrophin signaling pathway
- Non-small cell lung cancer
- Pancreatic cancer
- Pathways in cancer
- Prostate cancer
- Regulation of actin cytoskeleton
- Renal cell carcinoma
- Thyroid cancer
- Vascular smooth muscle contraction
- Related Disease
- Abnormalities, Multiple
- Adenocarcinoma
- Adenocarcinoma, Follicular
- Adenocarcinoma, Mucinous
- Adenocarcinoma, Papillary
- Adenoma
- Adenoma, Oxyphilic
- Adrenal Cortex Neoplasms
- Alcoholism
- Articulation Disorders
- Astrocytoma
- Brain Neoplasms
- Breast cancer
- Breast Neoplasms
- Carcinoma
- Carcinoma, Basal Cell
- Carcinoma, Medullary
- Carcinoma, Non-Small-Cell Lung
- Carcinoma, Papillary
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