商品介绍
- Specification
- Product Description:
- The RNA Prestain Marker High is a RNA ladder for precise sizing of RNA molecules in denaturing agarose gels. The RNA Prestain Marker High is a visible molecular weight marker for ssRNA, consisting of six colored (blue and purple) nucleic acids. The six colored bands (apparent molecular weights are 200, 500, 1,000, 2,000, 4,000 and 8,000 bases) are suitable for monitoring denaturing agarose gel electrophoresis and blotting onto membranes. The RNA Prestain Marker High shows the same mobility as that of the RNA Marker High (Cat # R0003) on denaturing agarose gel electrophoresis (>90% accuracy, see table 1). The RNA Prestain Marker High is supplied in a ready-to-use mixture without requiring heating or the addition of a denaturing agent before use.
- Quality Control Testing:
- After 24 hrs incubation of the RNA Prestain Marker High at 37°C, no visible degradation of the marker is observed in 1% agarose- 2.2 M formaldehyde gel electrophoresis.Electrophoresis profile of RNA Prestain Marker High (6 uL) on 1% agarose - 2.2 M forumaldehyde gel / 1 x MOPS buffer as running buffer.
- Recommend Usage:
- Recommended loading volumes:Comb (Load volume)4~6 mm (4~6 uL)>6 mm (>6 uL)
- Storage Buffer:
- In 40 mM MOPS, 10 mM AcONa, pH 7.0 (1 mM EDTA-2Na, 10% glycerol).
- Storage Instruction:
- Store at -80°C.Aliquot to avoid repeated freezing and thawing.
- Note:
- For accurate electrophoretic determination of molecular weights, the RNA Marker High (Cat # R0003) or RNA Marker High Easy (Cat # R0004) should be used.The migration of the RNA Prestain Marker High has been optimized to use 0.8-1.5% of agarose gel concentration (see table 1).Particularly avoid freeze-thaw cycle.It is not possible to use for acrylamide gel electrophoresis.
Table 1: Apparent molecular weights as a percentage compared to the RNA Marker High (Cat # R0003).
- Protocol:
Protocol Download
- Datasheet:
- Download
- Applications
- Electrophoresis
- Application Image
- Electrophoresis
品牌介绍
Abnova公司是专注于高通量蛋白表达和抗体制作，并将世界级的生物技术与台湾IT工业管理经验独特融于一体的全球性生物公司。Abnova通过基因组 学手段生产出的几万种重组蛋白和抗体，极大的扩展了生物试剂应用的视野和多样性。同时，Abnova还在不断的扩展其在系统应用中的产品线，包括成对抗 体、ELISA试剂盒、原位杂交荧光探针、无表达蛋白定量以及可用于蛋白翻译研究、诊断和制药公司应用的基础型体外诊断设备等。作为Abnova的授权代理商，艾美捷科技有限公司凭借丰富的专业经验，将一如既往的为中国区域客户提供Abnova优质的产品和技术服务。
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