商品介绍
- Specification
- Product Description:
- The RNA Marker Low consists of seven single-stranded RNAs. The 20-base and 50-base RNA are synthesized by chemically (not phosphorylated). The 100, 200, 300, 400 and 500 bases are synthesized by in vitro transcription. The RNA Marker Low is suitable for determinating size of single-stranded RNAs in denaturing polyacrylamide gel electrophoresis. The concentration of each RNA (20-500 base) in the marker is approximately 0.1 ug/ul. It is useful for estimating of RNA amount. The RNA Marker Low can be visualized by ethidium bromide staining.
- Quality Control Testing:
- After 18 hr incubation of the RNA Marker Low at 37°C, no visible degradation of the marker is observed in 5 % polyacrylamide / 8M urea gel electrophoresis.
- Recommend Usage:
- 1 uL (0.7 ug) RNA marker low mix with 5 uL of gel loading buffer.
- Storage Buffer:
- In 10 mM Tris-HCl (pH 8.0) buffer containing 1 mM EDTA.
- Storage Instruction:
- Store at -80 °C. Repeated freeze/thaw cycles should be avoided.
- Note:
- RNA is very sensitive to degradation by nucleases. To avoid damaging the RNA Marker Low, use extreme care during manipulations to prevent nuclease contamination. Wear gloves and use clean apparatus. Glassware should be pretreated with diethyl pyrocarbonate (DEPC). Nuclease-free disposable plasticware should be used. Solutions and reagents to mix the marker should be high grade and nuclease-free. To use, thaw the RNA Marker Low on ice and keep it on ice while using.
- Protocol:
Protocol Download
- Datasheet:
- Download
- Publication Reference
- 1.
- Hepatitis C virus genomic RNA dimerization is mediated via a kissing complex intermediate.Shetty S, Kim S, Shimakami T, Lemon SM, Mihailescu MR.RNA. 2010 May;16(5):913-25. Epub 2010 Apr 1.
- 2.
- Identification of small RNAs in Mycobacterium tuberculosis.Arnvig KB, Young DB.Mol Microbiol. 2009 Aug;73(3):397-408. Epub 2009 Jun 22.
- Applications
- Electrophoresis
- Application Image
- Electrophoresis
品牌介绍
Abnova公司是专注于高通量蛋白表达和抗体制作，并将世界级的生物技术与台湾IT工业管理经验独特融于一体的全球性生物公司。Abnova通过基因组 学手段生产出的几万种重组蛋白和抗体，极大的扩展了生物试剂应用的视野和多样性。同时，Abnova还在不断的扩展其在系统应用中的产品线，包括成对抗 体、ELISA试剂盒、原位杂交荧光探针、无表达蛋白定量以及可用于蛋白翻译研究、诊断和制药公司应用的基础型体外诊断设备等。作为Abnova的授权代理商，艾美捷科技有限公司凭借丰富的专业经验，将一如既往的为中国区域客户提供Abnova优质的产品和技术服务。
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