商品介绍
- Specification
- Product Description:
- The dsRNA Marker Easy is supplied in a ready-to-load mixture of loading dye (containing Tris-HCl buffer, glycerol, EDTA sodium salt, sodium chloride, bromphenol blue) and an ideal size marker for determinating sizes of double-stranded RNAs. The dsRNA Marker Easy consists of ten double-stranded RNAs, 10, 20, 30, 50, 100, 200, 300, 400, 500 and 1,000 base pairs. In 5 ul of the dsRNA Marker Easy, a 20 bp of dsRNA is approximately 50 ng. The dsRNA Marker Easy can be visualized by UV light after ethidium bromide staining.
- Quality Control Testing:
- After 18 hr incubation of the dsRNA Marker Easy at 37°C, no visible degradation of the marker is observed in 7.5 % polyacrylamide gel electrophoresis.
- Recommend Usage:
- 5 uL/lane
- Supplied Product:
- 6 x dsRNA Loading Buffer is used for preparation of dsRNA samples for non-denaturing polyacrylamide gel electrophoresis. One volume of 6 x dsRNA Loading Buffer is added to 5 volumes of sample. The 6 x dsRNA Loading Buffer is RNase free and contains Tris-HCl buffer (pH7.5), glycerol, EDTA sodium salt, bromphenol blue. Store at - 20 °C or - 80 °C.
- Storage Instruction:
- Store at -80 °C. Repeated freeze/thaw cycles should be avoided.
- Note:
- dsRNA is more resistant to RNase than ssRNA, dsRNA is sensitive to degradation by RNase. To avoid damaging the dsRNA Marker Easy, use care during manipulations to prevent nuclease contamination. Wear gloves and use clean apparatus. Glassware should be pretreated with diethyl pyrocarbonate (DEPC). Nuclease-free disposable plasticware should be used. Solutions and reagents to mix the product should be high grade and nuclease-free. To use, thaw the dsRNA Marker Easy on ice and keep it on ice while using
- Protocol:
Protocol Download
- Datasheet:
- Download
- Applications
- Electrophoresis
- Application Image
- Electrophoresis
品牌介绍
Abnova公司是专注于高通量蛋白表达和抗体制作，并将世界级的生物技术与台湾IT工业管理经验独特融于一体的全球性生物公司。Abnova通过基因组 学手段生产出的几万种重组蛋白和抗体，极大的扩展了生物试剂应用的视野和多样性。同时，Abnova还在不断的扩展其在系统应用中的产品线，包括成对抗 体、ELISA试剂盒、原位杂交荧光探针、无表达蛋白定量以及可用于蛋白翻译研究、诊断和制药公司应用的基础型体外诊断设备等。作为Abnova的授权代理商，艾美捷科技有限公司凭借丰富的专业经验，将一如既往的为中国区域客户提供Abnova优质的产品和技术服务。
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