商品介绍
- Specification
- Product Description:
- Recombinant human antibody raised against SARS-CoV/SARS-CoV-2 N.
- Reactivity:
- SARS-CoV, SARS-CoV-2
- Specificity:
- This antibody recognizes human SARS Coronavirus Nucleocapsid protein.
- Form:
- Liquid
- Purification:
- Protein A purification
- Purity:
- > 98% (SDS-PAGE)
- Isotype:
- IgG1
- Quality Control Testing:
- Antibody Reactive Against Recombinant Proteins.Western Blot analysis of different recombinant proteins were stained with this antibody at 1:1000 dilution. Lane 1: SARS-CoV-2 NP, Lane 2: SARS-CoV NP, Lane 3: MERS-CoV NP and Lane 4: HCoV-NL63 NP.
- Recommend Usage:
- ELISA (1:5000-1:20000)Flow Cytometry (1:500-1:1000)Immunofluorescence(1:500-1:1000)Western Blot (1:1000-1:5000)The optimal working dilution should be determined by the end user.
- Storage Buffer:
- In PBS, pH 7.4.
- Storage Instruction:
- Store at 4°C for at least 6 months. For long term storage, store at -20°C.Aliquot to avoid repeated freezing and thawing.
- Note:
- Multi-cycle kinetic analysis
Multi-cycle kinetic analysis of this antibody to human SARS-CoV-2 Nucleocapsid protein by using Biacore T200.
- Antibody Species:
- Human
- Source / Expression System:
- 293
- Datasheet:
Download
- Applications
- Western Blot (Transfected lysate)
- Western Blot analysis of Lane 1: HEK293 cells and Lane 2: transfected with SARS-CoV-2 VLP (Virus-like particle) were stained with this antibody at 1:1000 dilution.
- Western Blot (Recombinant protein)
- Immunofluorescence
- Immunofluorescent staining of 293T cells were respectively transfected with SARS-CoV, SARS-CoV-2, MERS-CoV and HCoV-NL63 nucleocapsid plasmid and stained with this antibody (1:500).
- Enzyme-linked Immunoabsorbent Assay
- ELISA assay of this antibody to SARS-CoV-2 nucleocapsid protein, SARS-CoV nucleocapsid protein, MERS-CoV nucleocapsid protein, HCoV-NL63 nucleocapsid protein and BSA (BSA: negative control).
- Flow Cytometry
- Application Image
- Western Blot (Transfected lysate)
- enlarge
- Western Blot (Recombinant protein)
- Immunofluorescence
- enlarge
- Enzyme-linked Immunoabsorbent Assay
- enlarge
- Flow Cytometry
品牌介绍
Abnova公司是专注于高通量蛋白表达和抗体制作，并将世界级的生物技术与台湾IT工业管理经验独特融于一体的全球性生物公司。Abnova通过基因组 学手段生产出的几万种重组蛋白和抗体，极大的扩展了生物试剂应用的视野和多样性。同时，Abnova还在不断的扩展其在系统应用中的产品线，包括成对抗 体、ELISA试剂盒、原位杂交荧光探针、无表达蛋白定量以及可用于蛋白翻译研究、诊断和制药公司应用的基础型体外诊断设备等。作为Abnova的授权代理商，艾美捷科技有限公司凭借丰富的专业经验，将一如既往的为中国区域客户提供Abnova优质的产品和技术服务。
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