商品介绍
- Specification
- Product Description:
- SARS-CoV-2 N (QHD43423.2) full-length recombinant protein with His tag at C-terminal expressed in Escherichia coli.
- Host:
- Escherichia coli
- Theoretical MW (kDa):
- 47.08
- Specificity:
- No non-specific binding to normal human serum was identified so far.
- Form:
- Liquid
- Preparation Method:
- Escherichia coli expression system
- Purification:
- Ni column
- Purity:
- > 95% by SDS-PAGE.
- Quality Control Testing:
- DTT-reducing SDS-PAGE Result
- Recommend Usage:
- AntigenELISAWestern BlotThe optimal working dilution should be determined by the end user.
- Storage Buffer:
- In 20 mM Tris, pH 8.5.
- Storage Instruction:
- This protein is not stable at 4°C in liquid. For long term storage store at -20 to -80°C.Aliquot to avoid repeated freezing and thawing.
- Datasheet:
Download
- Applications
- Western Blot
- Antigen
- Enzyme-linked Immunoabsorbent Assay
- ELISA plate was coated by N protein, 100 uL/well, at various concentrations. The direct ELISA analysis was performed by loading 100 uL per well of a anti C-ter His tag HRP conjugated mAb at a concentration of 1:2000. The plate was incubated for 1 hour at 37°C, then washed 5 time. Detection was performed using TMB substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2 M sulfuric acid. Absorbances were read on a spectrophotometer at 450 nm.
- SDS-PAGE
- Application Image
- Western Blot
- Antigen
- Enzyme-linked Immunoabsorbent Assay
- enlarge
- SDS-PAGE
品牌介绍
Abnova公司是专注于高通量蛋白表达和抗体制作，并将世界级的生物技术与台湾IT工业管理经验独特融于一体的全球性生物公司。Abnova通过基因组 学手段生产出的几万种重组蛋白和抗体，极大的扩展了生物试剂应用的视野和多样性。同时，Abnova还在不断的扩展其在系统应用中的产品线，包括成对抗 体、ELISA试剂盒、原位杂交荧光探针、无表达蛋白定量以及可用于蛋白翻译研究、诊断和制药公司应用的基础型体外诊断设备等。作为Abnova的授权代理商，艾美捷科技有限公司凭借丰富的专业经验，将一如既往的为中国区域客户提供Abnova优质的产品和技术服务。
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